behnaz bageshlooyafshar; Reza Rahchamani; Abdollah Mohammadi-Sangcheshmeh; Ehsan Seyedjafari; Yussof Mostafaloo
Volume 20, Issue 2 , August 2018, , Pages 339-349
Abstract
This study was conducted to investigate the differentiation potential of equine adipose-derived mesenchymal stem cell into bone in single-dimensional culture system (in plastic tissue culture) and in three-dimensional system (on poly-l-lactic acid scaffolds; PLLA). A porous structure that allows use ...
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This study was conducted to investigate the differentiation potential of equine adipose-derived mesenchymal stem cell into bone in single-dimensional culture system (in plastic tissue culture) and in three-dimensional system (on poly-l-lactic acid scaffolds; PLLA). A porous structure that allows use of three-dimensional distribution and provides optimal growth of cells is of great clinical significance in the field of tissue engineering. In current study using equine adipose-derived stem cells (ASCs), we intended to compare the osteogenic differentiation potential of PLLA nanofibrous scaffold with tissue culture plastic (TCP). Adipose tissues were collected from 3 adult horses, and ASCswere isolated by enzymatic digestion. PLLA nanofibrous scaffold was successfully prepared using a phase separation method. Viability and growth characteristics of ASCs on TCP and scaffold were investigated by tetrazolium (MTT) based colorimetric assay. Alizarin Red staining was performed for determination of calcium deposition following osteogenic differentiation. Furthermore, other common osteogenic markers such as alkaline phosphatase (ALP) activity, and calcium content were also analyzed. Our data showed that the PLLA scaffold had no detrimental effect on the cell growth rate as evaluated by MTT assay. However, ASCs that differentiated on PLLA nanofibrous scaffolds indicated higher ALP activity and more calcium content than that on TCP. Adequate proliferation rate and higher expression of osteogenic markers of stem cells cultured on PLLA nanofibrous scaffolds provide this scaffold as a suitable substrate to support proliferation and differentiation of ASCs in equine.
Seyyed Mojtaba Mousavi; Armin Tohidi; Mehdi Zhandi; Abdollah Mohammadi-Sangcheshmeh; Ghasem Amou-abediny
Volume 18, Issue 3 , October 2016, , Pages 593-601
Abstract
The effect of osmolarity and glycerol different levels in soybean lecithin-based extender on the bull sperm quality after cryopreservation was examined using six Holstein bulls in a 2 × 3 factorial trial based on completely randomized design, with three levels of osmolarity (250, 300 and 350 mOsml) ...
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The effect of osmolarity and glycerol different levels in soybean lecithin-based extender on the bull sperm quality after cryopreservation was examined using six Holstein bulls in a 2 × 3 factorial trial based on completely randomized design, with three levels of osmolarity (250, 300 and 350 mOsml) and two levels of glycerol (5 and 7 %) .In general, semen samples were collected 36 times (from each bull, six times). After the initial evaluation of semen, samples were mixed together and assigned to each of treatments. After freezing and thawing process, parameters that were evaluated including; motion characteristics by CASA, viability, plasma membrane integrity and morphology. The results showed that frozen-thawed sperm in treatment of G7P300 had higher values than the other groups for total motility (69.50 %), progressive motility (48.89 %), lateral head displacement (3.69 µm/s), curvilinear velocity (168.80 µm/s) and straightness coefficient (61.89 %) (P≤0.05). In the treatments containing seven and five percent of glycerol and osmotic pressure of 350 mOsml, plasma membrane integrity (23.14 and 25.63 %, respectively) and sperm viability (58.70 and 64.60 %, respectively) were lower compared to other treatments (P≤0.05). But, in terms of morphology, G7P350 (92.34 %) and G5P350 (92.57 %) treatments were better than other treatments. The results of these experiment showed that the extender contained of seven percentage of glycerol with osmalarity of 300 or 250 mOsml was more efficient for cryopreservation of Holstein bull sperm.
Javad Mohammad Moradi; Ali Akbar Khadem; Seyed Ahmad Hosseini; Arash Veshkini; Ali Asadi Alamouti; Abdollah Mohammadi-Sangcheshmeh
Volume 16, Issue 2 , October 2015, , Pages 75-83
Abstract
In vitro maturation (IVM) was carried out in the presence of different concentrations (10, 50, 100 or 200 µM) of α-linolenic acid (ALA). Embryonic cleavage, blastocyst formation following parthenogenetic activation (PA) and in vitro fertilization (IVF), and numbers of total and apoptotic ...
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In vitro maturation (IVM) was carried out in the presence of different concentrations (10, 50, 100 or 200 µM) of α-linolenic acid (ALA). Embryonic cleavage, blastocyst formation following parthenogenetic activation (PA) and in vitro fertilization (IVF), and numbers of total and apoptotic cells in blastocyst were then determine for the 50 μM concentration and compared with the control group. Out data revealed that ALA increased maturation (MII) rate as compared with control group (P<0.05) and oocytes in 200 μM ALA group showed a lower MII rate as compared with the control group. When oocytes treated with 50 μM ALA were subsequently used for PA or IVF, a higher (P<0.05) rate of blastocyst formation was observed and these embryos had a higher total cell number and a lower apoptotic cell number (P<0.05) as compared with the control group. In conclusion, our results show that supplementation of maturation medium with 50 μM ALA had a positive effect on meiotic maturation by increasing the MII rate and this in turn, stimulated blastocyst formation and also improved quality of the yielded blastocysts.